The use of Assay Defender® is always important when analyte and antibody meet and thus a high risk of interference effects is given.
Assay Defender® replaces the ordinary sample diluent.
Specimens should be diluted with Assay Defender® at least 1:2, if possible 1:10 (1 part specimen + 9 parts Assay Defender®).
Higher dilutions are not a problem, provided the analyte concentration is high enough.
Assay Defender® is also applicable for immuno-PCR and protein arrays as sample diluent.