Examples for use of Assay Defender®

The use of Assay Defender® is always important when analyte and antibody meet and thus a high risk of interference effects is given.

ELISA

Assay Defender® replaces the ordinary sample diluent.
Specimens should be diluted with Assay Defender® at least 1:2, if possible 1:10 (1 part specimen + 9 parts Assay Defender®).
Higher dilutions are not a problem, provided the analyte concentration is high enough.
Assay Defender® is also applicable for immuno-PCR and protein arrays as sample diluent.

 

use of Assay Defender in ELISA 1:2 dilution
1:2 dilution
use of Assay Defender in ELISA 1:10 dilution
1:10 dilution

Lateral Flow Assay

Assay Defender® replaces flow buffer or chase buffer.

use of Assay Defender in lateral flow assays

Bead-based Assays

Bead-based platforms with sample diluent

Assay Defender® replaces the ordinary sample diluent. Dilution depends on the analyte concentration.

use of Assay Defender in bead-based assays

Bead-based platforms without diluent

Assay Defender® replaces the storage buffers for capture and detection beads.

use of Assay Defender as bead storage buffer

Assay Defender® - Total HAMA and interference blocker

Assay Defender®  is a ready-to use assay diluent for reduction of interference caused by HAMA (human anti-mouse antibodies), rheumatoid factors, nonspecific binding, cross-reactivities and matrix effects.
Assay Defender® is particularly suitable for human or animal specimens…

Certification EN ISO 13485

Additionally to the EN ISO 9001 certification, CANDOR is now also certified according to EN ISO 13485.