Protein Array / Multiplex Assay
Protein arrays are miniaturized assays for detection of many proteins in parallel. They use proteins or antibodies immobilized on surfaces such as glass slides or beads. A single target protein is detected either by localization of a signal at a specific point in the array or by differences in the specific beads, which can be individually coded e.g. by color or fluorescence. The major advantage of this technique is that many analytes can be detected at the same time from one sample. But cross-talk between the multiplexed assays has to be avoided to enable sufficient reliability of results.
Multiplex applications are partially performed as bead-based assays. Different beads are coated with capture antibodies for different analytes. These beads are mixed with the sample for detecting several analytes in parallel. Multiplex assays are very susceptible to interferences and cross-reactivities. Due to the fact that several assay antibodies are measured simultaneously, it is necessary to avoid cross-reactivities e.g. by using LowCross-Buffer® as sample diluent.
Luminex Assays are often carried out with LowCross-Buffer®.
Following products can be used for Protein Arrays and Multiplex Assays:
Sample and antibody dilution buffer for immunoassays.
Sample diluent for blocking HAMA and other high affinity interfering antibodies. Minimizes nonspecific binding, cross-reactivities and matrix effects in immunoassays based on human or animal body fluids.
Coating and Wash Buffers
Coating Buffer 10x
Buffer for adsorptive immobilization of proteins and antibodies on surfaces.
Washing Buffer 10x
Wash Buffer for immunoassays. Available as TRIS or PBS buffer.
Surface Blockers and Coating Stabilizers
The Blocking Solution
Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.
BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
Liquid Plate Sealer®
Stabilizer for coated antibodies and antigens on polystyrene- or glass-surfaces.
Conjugate and Protein Stabilizers
Stabilizer for long-term storage of protein/antibodies at 2-8°C.
Diluent for long-term storage of HRP conjugates.
We would like to thank the CANDOR team for their comprehensive, friendly and flexible cooperation in the optimization of signal strength and the reduction of background in our membrane based human protein arrays.
With the appropriate combination of surface blocker and wash buffer made by CANDOR, we could finally not only reduce the serum background and stabilize the signal strength, but also standardize and simplify the handling in our production and for our customers.