Immuno-PCR combines ELISA technique with the exponential signal enhancement of a PCR (polymerase chain reaction). The specific antibodies are labeled with a DNA marker. By using the amplification steps of the PCR, the sensitivity can be better compared to the “classic” ELISA. Due to the fact that signals from nonspecific binding are significantly enhanced by the PCR-reaction, false-positive results are very common. As a consequence effective blocking of the plate surface and avoiding of interference are extremely important for Immuno-PCR.
The enhanced signals of this method also lead to higher background and interference. Therefore it is very important to eliminate background and interference effects. A combination of LowCross-Buffer® and The Blocking Solution can be used to avoid interferences.
For Immuno-PCR we offer following products:
Sample and antibody dilution buffer for immunoassays.
Sample diluent for blocking HAMA and other high affinity interfering antibodies. Minimizes nonspecific binding, cross-reactivities and matrix effects in immunoassays based on human or animal body fluids.
Coating and Wash Buffers
Coating Buffer 10x
Buffer for adsorptive immobilization of proteins and antibodies on surfaces.
Washing Buffer 10x
Wash Buffer for immunoassays. Available as TRIS or PBS buffer.
Surface Blockers and Coating Stabilizers
The Blocking Solution
Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.
BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
Liquid Plate Sealer®
Stabilizer for coated antibodies and antigens on polystyrene- or glass-surfaces.