ELISA
ELISA is the abbreviation for Enzyme-Linked Immuno Sorbent Assay.
The principle is the binding of a labeled specific antibody to the target antigen (called analyte). The signal of the enzyme labeled antibody is the conversion of a substrate, which can be detected and quantified.
The most important assay formats are:
Following products can be used for ELISA applications:
This pdf shows, how to use CANDOR products in a sandwich ELISA.
Assay Diluents
Assay Defender®
Sample diluent for blocking HAMA and other high affinity interfering antibodies. Minimizes nonspecific binding, cross-reactivities and matrix effects in immunoassays based on human or animal body fluids.
Surface Blockers and Coating Stabilizers
The Blocking Solution
Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.
SmartBlock™
BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
BSA-Block
Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
PlateBlock™
Animal-free and protein-free blocker for plastic surfaces of ELISA plates specifically for serological assays (antigen-capture-assays).
Liquid Plate Sealer®
Stabilizer for coated antibodies and antigens on polystyrene- or glass-surfaces.