Immuno-PCR combines ELISA technique with the exponential signal enhancement of a PCR (polymerase chain reaction). The specific antibodies are labeled with a DNA marker. By using the amplification steps of the PCR, the sensitivity can be better compared to the “classic” ELISA. Due to the fact that signals from nonspecific binding are significantly enhanced by the PCR-reaction, false-positive results are very common. As a consequence effective blocking of the plate surface and avoiding of interference are extremely important for Immuno-PCR.
The enhanced signals of this method also lead to higher background and interference. Therefore it is very important to eliminate background and interference effects. A combination of LowCross-Buffer® and The Blocking Solution can be used to avoid interferences.
For Immuno-PCR we offer following products: