Stripping Buffer

Product description

pH-value: pH 2,8 ± 0,2
Preservative: contains 0.1% ProClin® 300
Storage: shelf life at –20 °C: 1 year shelf life at 2 – 8 °C: 6 months

For research use only, not for diagnostic use

Available package sizes:

50   ml order number 150 050
125 ml order number 150 125
500 ml order number 150 500

Instructions for use

Ready-to-use

Stripping Buffer removes reaction solution, primary and secondary antibodies from Western blotting membranes. After stripping the membrane can be used for repeated detection (reprobing) with antibodies.

Membrane is incubated in a vessel with Stripping Buffer for removing the antibodies. For this purpose the membrane is gently shaked in Stripping Buffer for 30 – 60 minutes at room temperature.

After stripping the membrane has to be washed with Washing Buffer and can be used for repeated detection.
If you detect with alkaline phosphatase, the wash buffer should not contain phosphates.

Important: The designated incubation conditions are standard values, which have to be adopted by the user. Incubation times are depending on characteristics of the used antibodies.

ProClin is a registered trade mark of Rohm and Haas Company.