Sample Buffer
Application note
Sample Buffer
Product description
pH-value: pH 7,2 ± 0,2
Preservative: contains 0.1% ProClin® 300
Storage: shelf life at –20 °C, 1 year – tolerates repeated freezing and thawing cycles
shelf life at 2 – 8 °C, 6 months
For research use only, not for diagnostic use
Available package sizes:
50 ml order number 105 050
125 ml order number 105 125
500 ml order number 105 500
Instructions for use
Sample Buffer is a dilution buffer for specimen and antibodies für direct use in immunoassays. Antibodies, coupled with alkaline phosphatase or peroxidase, can be diluted and used in Sample Buffer without problems, because Sample Buffer is free of phosphate.
Applications include ELISA, EIA, Western blotting, immuno-PCR and protein arrays. Sample Buffer is not siutable for electrophoresis.
Immediately before use the buffer should be mixed thoroughly. Sample Buffer is ready-to-use. Repeated freezing and thawing is possible. Specimen with the analyte – as well as the detection antibody – is diluted in Sample Buffer and then used in the assay. It is necessary to treat standards and specimen in the same way!
Sample Buffer is designed for trouble free assays without interference. If you have background or false-positives – for example derived from cross reactivities or matrix effects – we recommend to use LowCross-Buffer® rather than Sample Buffer.
ProClin is a registered trade mark of Rohm and Haas Company.
Sample Buffer without detergents
Antibody dilution buffer for immunohistochemistry
Product description
pH-value: pH 7,2 ± 0,2
Preservative: contains 0.1% ProClin® 300
Storage: shelf life at –20 °C, 1 year – repeated freezing and thawing cycles possible shelf life at 2 – 8 °C, 6 months
For research use only, not for diagnostic use
Available packaging size:
50 ml order number 106 050
125 ml order number 106 125
500 ml order number 106 500
Instructions for use
Sample Buffer is a dilution buffer for specimen and antibodies für direct use in immunoassays. Antibodies, coupled with alkaline phosphatase or peroxidase, can be diluted and used in Sample Buffer without problems, because Sample Buffer is free of phosphate. Sample Buffer without detergents contains neither Tween nor other detergents. Thus it is for use expecially in immunohistochemistry, where detergents sometimes can make problems in detection.
Immediately before use the buffer should be mixed thoroughly. Sample Buffer is ready-to-use. Repeated freezing and thawing is possible. Primary and secondary antibodies can be diluted directly in Sample Buffer.
Sample Buffer is designed for trouble free assays without interference. If you have background, we recommend to use LowCross-Buffer® rather than Sample Buffer.
ProClin is a registered trade mark of Rohm and Haas Company.
Antibody dilution buffer for immunohistochemistry
Product description
pH-value: pH 7,2 ± 0,2
Preservative: contains 0.1% ProClin® 300
Storage: shelf life at –20 °C, 1 year – repeated freezing and thawing cycles possible shelf life at 2 – 8 °C, 6 months
For research use only, not for diagnostic use
Available packaging size:
50 ml order number 106 050
125 ml order number 106 125
500 ml order number 106 500
Instructions for use
Sample Buffer is a dilution buffer for specimen and antibodies für direct use in immunoassays. Antibodies, coupled with alkaline phosphatase or peroxidase, can be diluted and used in Sample Buffer without problems, because Sample Buffer is free of phosphate. Sample Buffer without detergents contains neither Tween nor other detergents. Thus it is for use expecially in immunohistochemistry, where detergents sometimes can make problems in detection.
Immediately before use the buffer should be mixed thoroughly. Sample Buffer is ready-to-use. Repeated freezing and thawing is possible. Primary and secondary antibodies can be diluted directly in Sample Buffer.
Sample Buffer is designed for trouble free assays without interference. If you have background, we recommend to use LowCross-Buffer® rather than Sample Buffer.
ProClin is a registered trade mark of Rohm and Haas Company.
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