LowCross-HRP®

Product description

LowCross-HRP® is a dilution buffer and storage solution for peroxidase-conjugates!
pH-value: pH 7.2 ± 0.2
Preservative: contains 0.1% ProClin® 300
Storage: shelf life at 2 – 8 °C: 24 months – does not tolerate freezing

For research use only, not for diagnostic use

Available Package sizes:

50  ml order number 200 050
125 ml order number 200 125
500 ml order number 200 500

Instructions for use

LowCross-HRP® is a dilution buffer for long-term storage of peroxidase-conjugates. This can be antibody-peroxidase-conjugates as well as Neutravidin- or Streptavidin-peroxidase-conjugates. Conjugates can be diluted directly in LowCross-HRP® in end-user concentrations. Typical concentrations of conjugates are in between 40 and 500 ng/ml.

Immediately before use the buffer should be mixed thoroughly.

LowCross-HRP® is a combination of LowCross-Buffer® and a modern stabilizer for stabilizing antibodies and peroxidase. Therefore you get long-term stabilization as well as the LowCross-Buffer® effect in one solution.

The LowCross-Buffer® effect:
LowCross-Buffer® was specially developed to minimise cross reactivities, nonspecific binding and matrix effects in immunoassays. Interference derived from HAMA (human anti-mouse antibodies) rheumatoid factors or other endogenous interfering factors can be reduced significantly.

Appearance of signal reduction:
In some cases a smooth reduction of the wanted signal can be observed. LowCross-Buffer® reduces low- and medium-affinity binding. That means that by using low- and medium-affinity detector-antibodies or polyclonal detector-antibodies a smooth reduction of signals can appear. Polyclonal antibodies normally contain low- and medium-affinity binding components. In the case of polyclonal antibodies a moderate increase of the concentration of the antibody can lead to the previously seen signals.

HRP – long-term stabilization of peroxidase:
LowCross-HRP® contains stabilizing components for antibodies and for peroxidase. This makes LowCross-HRP® the ideal solution, if you want to store peroxidase-conjugates for a long period in a ready-to-use solution. Dilute the conjugates directly in LowCross-HRP®.

Testing LowCross-HRP® with any new assay is recommended.

Stability data of one peroxidase-conjugate cannot be transferred to other conjugates. Therefore every conjugate has to be tested for its shelf life in LowCross-HRP®. If LowCross-HRP® is used for immunodiagnostic kits, the shelf life has to be tested according to the current European directive for in-vitro-diagnostics or according to corresponding rules, which apply.

LowCross-HRP and LowCross-Buffer are registered trademarks of CANDOR Bioscience GmbH. ProClin is a registered trade mark of Rohm and Haas Company.