Literature

translated article of Laborwelt published in No. 4/2005 - Vol. 6
original article: "Assayoptimierung: Störeffekte bei Immunoassays erkennen und vermeiden"


Dr. Peter Rauch, Angela Zellmer, CANDOR Bioscience GmbH, Münster
Dipl.-Chem. Nico Dankbar, Institute of analytical chemistry, University of Münster Dr. Christoph Specht, PARA Bioscience GmbH, Gronau
Dr. Detlef Sperling, MACHEREY-NAGEL GmbH & Co. KG, Düren

Immunoassays are analytical laboratory methods for the detection of different substances using antibodies. They are used commonly in bioanalytical and biochemical laboratories of the Life Science industry. The methods are Enzyme-linked immunosorbent assays (ELISA) and Enzyme immunoassays (EIA), Western blotting, Radio immunoassays (RIA), Protein Arrays, immunohistochemistry or also the immuno polymerase chain reaction (immuno-PCR). In all kinds of immunoassays the detection of the analyte can be influenced by interference. Very frequently cross reactivities, unspecific binding and matrix effects occur. Interfering substances are present in more or less significant concentrations in real specimen and interact with the analytes or with the capture respectively the detector antibodies directly. By application of a new buffer (LowCross-Buffer®) instead of the usually used buffers most interference effects can be avoided. Through this exchange the quality of the assays and the efficiency of the assay development is improved.

Full article 


Genetic Engineering & Biotechnology News Jul 1 2008 (Vol. 28, No. 13)
Assay: Avoiding Interference in Immunoassays

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