FAQ

Question

Can LowCross-Buffer® be used with monoclonal and polyclonal antibodies?

Answer

Yes. But if you use polyclonal antibodies you’ve to notice the composition of polyclonal antibodies consisting of many antibodies with different affinities. LowCross-Buffer® only allows high affinity binding whereas low and medium affinity binding is reduced.
If you use LowCross-Buffer® it can be necessary to increase the concentration of the polyclonal detection antibody moderately. The increase of the antibody concentration also increases the concentration of high affinity antibodies, while LowCross-Buffer® inhibits binding of low and medium affinity antibodies.
Whether you have to increase the concentration of antibodies in the assay or not depends on the quality of the antibodies.