FAQ

Question

I get a high background in my sandwich ELISA which is detected by a peroxidase labelled secondary antibody. What can be the reason and can LowCross-Buffer® help?

Answer

It’s important to know if the secondary antibody was...

Question

Do I have to dilute LowCross-Buffer®?

Answer

No. LowCross-Buffer® is ready to use.
Sample or...

Question

Can buffer components influence the colour reaction of enzymatic detection?

Answer

LowCross-Buffer® doesn’t influence the enzymatic...

Question

Can I use LowCross Buffer® in combination with fluorescent dyes e.g. in protein arrays?

Answer

Yes. For protein arrays there are positive results....

Question

Can LowCross-Buffer® be used with monoclonal and polyclonal antibodies?

Answer

Yes. But if you use polyclonal antibodies you’ve to...

Question

How often can I freeze and thaw LowCross-Buffer®?

Answer

It’s no problem to freeze and thaw the buffer...

Question

Why does LowCross-Buffer® reduce matrix effects?

Answer

Most of the so called matrix effects are based on...

Question

How do I have to use LowCross-Buffer®?

Answer

LowCross-Buffer® is a dilution buffer for specimens...

Question

What is specific to LowCross-Buffer®?

Answer

LowCross-Buffer® is a newly developed sample buffer...

Question

Can LowCross-Buffer® replace a blocking solution for surfaces (e.g. Western blotting membranes, ELISA plates)?

Answer

No! LowCross-Buffer® is no blocking buffer for...

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