Coating Buffer
Application note
Coating Buffer pH 7,4
Product description
Composition: PBS based buffer 10x stock solution
pH-value: pH 7,4 ± 0,2
Preservative: Buffer is delivered without any preservatives, because some preservatives can interfere with the process of coating. Thus coating buffer is safe and easy useable for many applications.
Storage: 10x stock solution: shelf life at –20 °C, 1 year shelf life at 2 – 8 °C, 6 months
Use working solution immediately!
For research use only, not for diagnostic use
Available package sizes:
125 ml order number 120 125
500 ml order number 120 500
Instructions for use
Coating Buffer pH 7,4 is designed for adsorptive immobilisation of proteins and antibodies on plastics surfaces (for example microtiterplates) or other protein binding surfaces. Applications include ELISA, EIA, RIA, protein arrays and immuno-PCR.
Crystals of salt can precipitate after storage at 2 – 8 °C or after freezing. Therefore Coating Buffer must be warmed up to room temperature and should be mixed thoroughly before preparing the working solution. This leads to dissolving of salt after shaking. Stock solution is diluted 1:10 with salt free water to get the working solution. Use working dilution immediately.
The proteins or antibodies for immobilisation are diluted in this working solution and used after mixing. Typical concentration range for standard ELISA is in between 0,5 µg/ml and 2 µg/ml for capture antibodies.
Depending on surface as well as on proteins or antibodies the useful incubation times can vary. Consequently, individual optimisation of the incubation procedure is recommended. Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilisation. Select from Coating Buffer pH 7,4 and pH 9,6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilisation.
For the optimisation of the coating procedure of a newly developed immunoassay we strongly recommend testing the two Coating Buffers side by side.
Coating Buffer pH 9,6
Product description
Composition: Carbonate based buffer 10x stock solution
pH-value: pH 9,6 ± 0,2
Preservative: Buffer is delivered without any preservatives, because some preservatives can interfere with the process of coating. Thus coating buffer is safe and easy useable for many applications.
Storage: 10x stock solution: shelf life at –20 °C, minimum 3 months shelf life at 2 – 8 °C, 1 month
Use working solution immediately!
For research use only, not for diagnostic use
Available package sizes:
125 ml order number 121 125
500 ml order number 121 500
Instructions for use
Coating Buffer pH 9,6 is designed for adsorptive immobilisation of proteins and antibodies on plastics surfaces (for example microtiterplates) or other protein binding surfaces. Applications include ELISA, EIA, RIA, protein arrays and immuno-PCR.
Crystals of salt can precipitate after storage at 2 – 8 °C or after freezing. Therefore Coating Buffer must be warmed up to room temperature and should be mixed thoroughly before preparing the working solution. This leads to dissolving of salt after shaking. Stock solution is diluted 1:10 with salt free water to get the working solution. Use working dilution immediately.
The proteins or antibodies for immobilisation are diluted in this working solution and used after mixing. Typical concentration range for standard ELISA is in between 0,5 µg/ml and 2 µg/ml for capture antibodies.
Depending on surface as well as on proteins or antibodies the useful incubation times can vary. Consequently, individual optimisation of the incubation procedure is recommended. Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilisation. Select from Coating Buffer pH 7,4 and pH 9,6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilisation.
For the optimisation of the coating procedure of a newly developed immunoassay we strongly recommend testing the two Coating Buffers side by side.
Product description
Composition: Carbonate based buffer 10x stock solution
pH-value: pH 9,6 ± 0,2
Preservative: Buffer is delivered without any preservatives, because some preservatives can interfere with the process of coating. Thus coating buffer is safe and easy useable for many applications.
Storage: 10x stock solution: shelf life at –20 °C, minimum 3 months shelf life at 2 – 8 °C, 1 month
Use working solution immediately!
For research use only, not for diagnostic use
Available package sizes:
125 ml order number 121 125
500 ml order number 121 500
Instructions for use
Coating Buffer pH 9,6 is designed for adsorptive immobilisation of proteins and antibodies on plastics surfaces (for example microtiterplates) or other protein binding surfaces. Applications include ELISA, EIA, RIA, protein arrays and immuno-PCR.
Crystals of salt can precipitate after storage at 2 – 8 °C or after freezing. Therefore Coating Buffer must be warmed up to room temperature and should be mixed thoroughly before preparing the working solution. This leads to dissolving of salt after shaking. Stock solution is diluted 1:10 with salt free water to get the working solution. Use working dilution immediately.
The proteins or antibodies for immobilisation are diluted in this working solution and used after mixing. Typical concentration range for standard ELISA is in between 0,5 µg/ml and 2 µg/ml for capture antibodies.
Depending on surface as well as on proteins or antibodies the useful incubation times can vary. Consequently, individual optimisation of the incubation procedure is recommended. Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilisation. Select from Coating Buffer pH 7,4 and pH 9,6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilisation.
For the optimisation of the coating procedure of a newly developed immunoassay we strongly recommend testing the two Coating Buffers side by side.
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