Coating Buffer 10x
Buffer for adsorptive immobilization of proteins and antibodies on plastic surfaces (for example microtiter plates) or other protein binding surfaces
Depending on the protein or the antibody the pH of the Coating Buffer is crucial for efficient immobilization. Select from Coating Buffer pH 7.4 and pH 9.6, as the pH-value can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilization.
For the optimization of the coating procedure of a newly developed immunoasay we strongly recommend testing the two Coating Buffers side by side. Depending on the surface as well as on proteins or antibodies the required incubation time can vary.
Consequently, individual optimization of the incubation procedure is recommended.
Simply dilute with water!
For research use only, not for diagnostic use
Available package sizes:
Coating Buffer pH 7.4 (based on PBS) 125 ml
order number 120 125
Coating Buffer pH 7.4 (based on PBS) 500 ml
order number 120 500
Coating Buffer pH 9.6 ((based on carbonate) 125 ml
order number 121 125
Coating Buffer pH 9.6 (based on carbonate) 500 ml
order number 121 500