Western Blot / Line Blot

The Western blotting technique is used to detect proteins from crude mixtures. First, the proteins are separated by gel-electrophoresis into bands. Then all proteins are transferred to a membrane, where the target protein band is detected with primary antibodies specific to the target protein. Such primary antibodies on the membrane are detected with secondary antibodies, which are either enzymatic or fluorescence labeled.

For Western Blot/Line Blot we offer following products:

ReadyTector®

ReadyTector®

The all-in-one Solution for Western Blotting

Assay Diluents

LowCross-Buffer®

Assay diluent for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays.

Sample Buffer

Sample and antibody dilution buffer for immunoassays.

Blockers

The Blocking Solution

Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.

SmartBlock™

BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

BSA-Block

Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

Stripping and Wash Buffers

Stripping Buffer

Buffer for stripping antibodies from membranes for multiple reprobing.

Washing Buffer 10x

Wash Buffer for immunoassays. Available as TRIS or PBS buffer.

Conjugate and Protein Stabilizers

Antibody Stabilizer

Stabilizer for long-term storage of protein/antibodies at 2-8°C.

HRP-Protector™

Diluent for long-term storage of HRP conjugates.

LowCross® HRP-Stab

Diluent for long-term storage of HRP conjugates and for minimizing nonspecific binding.

 

 

 

CANDOR Bioscience GmbH
Simoniusstrasse 39
88239 Wangen
Tel.: +49 (0) 75 22 - 7952 70
Fax: +49 (0) 75 22 - 7952 729
E-Mail: info@candor-bioscience.de
Web: www.candor-bioscience.de