Western Blot / Line Blot
The Western blotting technique is used to detect proteins from crude mixtures. First, the proteins are separated by gel-electrophoresis into bands. Then all proteins are transferred to a membrane, where the target protein band is detected with primary antibodies specific to the target protein. Such primary antibodies on the membrane are detected with secondary antibodies, which are either enzymatic or fluorescence labeled.
For Western Blot/Line Blot we offer following products:
Surface Blockers
The Blocking Solution
Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.
SmartBlock™
BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.
BSA-Block
Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.