Immuno-PCR

Immuno-PCR combines ELISA technique with the exponential signal enhancement of a PCR (polymerase chain reaction). The specific antibodies are labeled with a DNA marker. By using the amplification steps of the PCR, the sensitivity can be better compared to the “classic” ELISA. Due to the fact that signals from nonspecific binding are significantly enhanced by the PCR-reaction, false-positive results are very common. As a consequence effective blocking of the plate surface and avoiding of interference are extremely important for Immuno-PCR.

The enhanced signals of this method also lead to higher background and interference. Therefore it is very important to eliminate background and interference effects. A combination of LowCross-Buffer® and The Blocking Solution can be used to avoid interferences.

For Immuno-PCR we offer following products:

Assay Diluents

LowCross-Buffer®

Assay diluent for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays.

Sample Buffer

Sample and antibody dilution buffer for immunoassays.

Coating and Wash Buffers

Coating Buffer 10x

Buffer for adsorptive immobilization of proteins and antibodies on surfaces.

Washing Buffer 10x

Wash Buffer for immunoassays. Available as TRIS or PBS buffer.

 

 

Blockers and Coating Stabilizers

The Blocking Solution

Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.

SmartBlock™

BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

BSA-Block

Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

Liquid Plate Sealer®

Stabilizer for coated antibodies and antigens on polystyrene- or glass-surfaces.

 

 

Conjugate and Protein Stabilizers

Antibody Stabilizer

Stabilizer for long-term storage of protein/antibodies at 2-8°C.

CANDOR Bioscience GmbH
Simoniusstrasse 39
88239 Wangen
Tel.: +49 (0) 75 22 - 7952 70
Fax: +49 (0) 75 22 - 7952 729
E-Mail: info@candor-bioscience.de
Web: www.candor-bioscience.de