ELISA

ELISA is the abbreviation for Enzyme-Linked Immuno Sorbent Assay.

The principle is the binding of a labeled specific antibody to the target antigen (called analyte). The signal of the enzyme labeled antibody is the conversion of a substrate, which can be detected and quantified. 

The most important assay formats are:

Sandwich ELISA- Antibody Coated

Sandwich ELISA - Antigen Coated

Typical format in virology or allergy diagnostics e.g. for detection of specific IgG, IgE or IgM in human serum. This assay format is also known as Antigen-Down format.

Competitive ELISA - Antibody Coated

This type of assay is based on the competition between the analyte of interest and the tracer for a limited number of specific antibody binding sites.

Following products can be used for ELISA applications:

Assay Diluents

LowCross-Buffer®

Assay diluent for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays.

Sample Buffer

Sample and antibody dilution buffer for immunoassays.

Coating and Wash Buffers

Coating Buffer 10x

Buffer for adsorptive immobilization of proteins and antibodies on surfaces.

Washing Buffer 10x

Wash Buffer for immunoassays. Available as TRIS or PBS buffer.

Blockers and Coating Stabilizers

The Blocking Solution

Casein based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces.

SmartBlock™

BSA-free blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

BSA-Block

Standard blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces.

Liquid Plate Sealer®

Stabilizer for coated antibodies and antigens on polystyrene- or glass-surfaces.

Conjugate and Protein Stabilizers

Antibody Stabilizer

Stabilizer for long-term storage of protein/antibodies at 2-8°C.

HRP-Protector™

Diluent for long-term storage of HRP conjugates.

LowCross® HRP-Stab

Diluent for long-term storage of HRP conjugates and for minimizing nonspecific binding.

 

 

 

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